Molecular Vision: Szaflik, Mol Vis 2008; 14:1713-1718. Table 1

Table 1. Primers for polymerase chain reaction amplification and sequencing of KRT3 and KRT12, their annealing temperature (Ta), and expected amplicon size.

Gene/exon	Forward primer sequence (5’→ 3’)	Reverse primer sequence (5’→ 3’)	Ta (°C)	Product size (bp)
KRT3
exon 1	TgCACAggTCTTCATTTCCCATCC	TCCTCAACCCTggATATCTTCCCA	61	887
exon 2	AgTgTTgCCTgATgTTgCTTCCTg	ACCATgCTTggAgAAggAAggTgA	61	439
exon 3	ATggAgggAgggAAgAgATgAACT	ATTgCTCCAAAggCCTGAACTTgg	61	275
exon 4	gCTCTTTCTTgCTgCAgTTgTggT	gCACCAgCCTCAAATCTggAAACA	60	238
exon 5	AgTgAACAAgCTCCCTCTgTgTTg	TgAAACCTCCAgTggATCCCgTAA	60	235
exon 6	AAggTTTggTgggTgATgTTggAg	ATTTgTggAgATACTgCCCTgTgg	61	345
exon 7	AATCCATTgCATgTCAggAAgggC	TATCTGGCCCTTGGCCTATGACTT	60	354
exon 8	TgTTggTgATgTgCTTTgTgACgg	AAgCCAATCACTTCCCTCTCCTCT	60	228
exon 9	ACAATAACATAgCAgCTggCCTgg	AATACTCAgAggCCCggAgTgAAA	61	756
KRT12
exon 1a	AgTgAACTTTTCAACTgCgA	TgCCCgAgAgAATACCTAgA	61.5	450
exon 1b	AggACTgggTgCTggTTAT	CTgCAAgTACAgCTAAATTggA	62	447
exon 2	TAgggCTTCAATCTTgTgTgTgTCCC	TTTATATCAATgAAggCAggACAgTAggAC	61.2	200
exon 3	CCCTCAACTgCTTTgCACTTggTT	CTCCATACTTgTCCTgACTCCAgA	58.4	289
exon 4	CAgggCCCACgAAAgTCACAAT	gTTCgCAggCCTTTCTgTgAATgT	58.3	272
exon 5	ACATTCACAgAAAggCCTgCgAAC	TggAAgTCCAAAggATgCTACgTC	58.4	235
exon 6	gCTCgTgCgCAAACAgACgT	CCCAggCATATCTTTACTAgA	60	490
exon 7	AgCCACCTgAACCACCTACTCTAA	AgCTATgAggTTACAggCATgAgC	63	469
exon 8	gCCTACATTAAACAACCAgTgTTgg	CAAgCAATCATCTTgCCTCTCAgC	61	684