Figure 6 of Wu, Mol Vis 2008; 14:1705-1712.

Figure 6. Western blot assay of proteins involved in apoptosis in corneal endothelial cells. Following incubation of cells either in the absence of mitomycin C for 24 h (labeled as C in the image) or in the presence of 0.01 mg/ml (labeled as 0.01 in the image) and 0.001 (labeled as 0.001 in the image) mg/ml mitomycin C for 24 h, cells were subjected to SDS–PAGE electrophoresis and immunoblotting using antisera against Bcl-2, p53, and p21. Densitometric analysis of protein bands showed the optical density values of various proteins with a detailed description in the Results. The respective control values of three proteins were assumed as 100% response. The optical density of the Bcl-2 protein decreased to 64%±4% at 0.01 mg/ml and 86%±5% at 0.001 mg/ml in comparison with the control protein value of 100%±4%. The p53 protein only significantly increased at 0.01 mg/ml to 139%±4% over the control value. The p21 protein increased to 373%±6% at 0.01 mg/ml and 149%±7% at 0.001 mg/ml in comparison with the control value. Two other independent experiments produced similar results.