Figure 4. Apoptotic DNA characteristics of
corneal endothelial cells visualized with TUNEL staining. Cells were
incubated either in the absence of mitomycin C for 15 h and 24 h
(control, A) or in the presence of 0.001 mg/ml MMC for 24 h (B)
and 0.01 mg/ml mitomycin-C for 15 h (C). The typical positive
DNA apoptosis was clearly stained following exposure to mitomycin C.
After exposure of cells to 0.001 mg/ml mitomycin C, cellular chromatin
condensed and marginalized at the nuclear membrane. After exposure to
0.01 mg/ml mitomycin C, cells were seriously damaged and formed typical
apoptotic bodies containing cytosol, condensed chromatin, and
organelles. Each bar represents 3 μM. Two other independent experiments
produced similar results.