Figure 3. Immunofluorescent staining of cytochrome c in cultured corneal endothelial cells. Cells were incubated either in the absence of mitomycin C for 24 h (control, A) or in the presence of 0.001 mg/ml (B) and 0.01 mg/ml (C) mitomycin C for 24 h. The intensity of fluorescence staining with cytochrome c was gradually enhanced from the control cells to the apoptotic cells. Apoptotic changes throughout the cytosol were clearly visible following exposure to 0.01 mg/ml mitomycin C for 24 h. The bar in each panel represents 5 μm. Two other independent experiments produced similar results.