The relationship between diabetic retinopathy (DR) and its affected factors were tested by multivariable logistic regression analysis. The presence of DR was regarded as the dependent variable, and independent variables included genotype of EDN1, eNOS, ITGA, and LTA, gender, age, age at onset of diabetes, HbA1c, therapy of insulin, hypertension, and hyperlipidemia. Only items with p values <0.05 were listed. After adjustment for age, age at onset of diabetes and insulin therapy, etc, EDN1 Asn/Asn was the only independent protective factor for DR and younger onset age of diabetes was a significant risk factor for nonproliferative diabetic retinopathy (NPDR) progressing to proliferative diabetic retinopathy (PDR).