Figure 2. Confocal images of the sclera at the end of the 24 h nanoparticle (20 nm) transport study. A shows the control fluorescence and combined fluorescence while B shows the phase contrast images. C and D are nanoparticle exposed tissue fluorescence and combined fluorescence and phase contrast images, respectively. In each image, the scleral (donor) side is on the left and the vitreal (receiver) side is on the right. The particles are concentrated on the outer edge of the sclera. There are very few or no particles on the vitreal side of the tissue.