Figure 4. Retinal expression of p27^Kip1 in retina evaluated through immunohystochemistry assay. In (A), we can see a representative immunohistochemical staining for p27^Kip1 in retina of control and diabetic Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). The brownish color represents staining for p27^Kip1, and the blue color represents the staining of retinal tissue with hematoxylin. Original magnification 1000x, the bar in the figure represents a scale of 50µm. The graphs represents the score of positivity of p27^Kip1 in retinal slides expressed by mean±SD in ganglion cell (B) and inner nuclear (C) layers. Asterisk (*) is p=0.05 versus CT-WKY and CT-SHR, and dagger (†) is p=0.02 versus CT-SHR.