Figure 2. Immunofuorescence assay for double labeling of BrDU positive cells in retinal sections against glial, neuron and endothelial markers. In panel A, it is shown a retinal section showing two cells labeled for glial fibrillar acidic protein (GFAP) and BrdU in the ganglion cell layer. In B, there are the presence of two BrdU-positive cells in outer nuclear layer of the retina co-stained with GFAP antibody. In this slide (C), the BrDU positive cell is stained for nestin in the inner nuclear (long arrows) and ganglion cell (short arrows) layers of the retina. For identification of amacrine/bipolar origin of BrDu positive cell in the retina, an immunofluorescence for PKC- α antigen was performed. There is a BrDU positive cell that also stained for PKC- α in the outer nuclear (D). In panel E, elongated endothelial cells were identified expressing both Flk-1 and BrdU, localized in theinner nuclear layer of the retina