Figure 3. Electrophoretic mobility assays of PCR-RFLP and sequence results. A: PCR products were separated on 12% polyacrylamide gel from three representative samples, and the genotypes are shown for (1) Arg76Lys (227G→A), (2) Pro13Leu (38 C→T), and (3) Gln337Stop (1009C del). The sizes of the molecular weight marker and DNA fragments are shown in the left and the right sides of the gel, respectively. B: The representative chromatogram contains the sequence from the mutant DNA sequence strand for (1) Arg76Lys and (2) Pro13Leu as well as from the (3) wild-type and (4) mutant DNA sequence of Gln337Stop.