Figure 5. Colocalization of SMAD3 and phospho-SMAD3 protein in human corneal epithelial cells. FITC marked the secondary antibody (green; left), and PI dyed the nucleus (red; middle). Merged images were showed at the right of A and B. Both SMAD3 (A) and phospho-SMAD3 (B) were more weakly expressed at 12 h and 24 h p.i. compared to the uninfected cells. Scale bar: 10 μm.