Figure 2. Reverse transcription polymerase chain reaction analysis of the TGF-β isoforms in human corneal epithelial cells infected with HSV-1. A: Products of RT-PCR that were run on 2% agarose gel electrophoresis. The intensities of TGF-β1 bands decreased significantly at 8 h, 12 h, and 24 h p.i., while that of TGF-β2 and TGF-β3 bands unchanged. GAPDH was used as an internal control. B: The level of mRNA was expressed as the ratio of integrated optical density (IOD) of specific PCR products over GAPDH IOD. Each data was the mean value of three independent experiments. Single asterisks indicate significant differences (p<0.05).