Figure 1. Expression of M6a in mouse retinas from various developmental stages. A: Semiquantitative RT–PCR for M6a using total RNA extracted from mouse retinas at various developmental stages. Glyceraldehyde-3-phosphate dehydrogenase (G3PDH) was used as the control. B-E: Immunostaining of M6a in frozen sections of mouse retina from various developmental stages. Coimmunostaining was performed with anti-M6a and anti-βIII-tubulin (C), anti-glutamine synthetase (D), or antisynaptophysin (E) antibodies. The right two columns are magnified figure of the square area indicated by broken lines in left two columns B. The scale bar represents 100 μm. The following abbreviations are used in this figure: inner plexiform layer (IPL); outer plexiform layer (OPL); ganglion cell layer (GCL); neuroblastic layer (NBL); inner nuclear layer (INL); outer nuclear layer (ONL); retinal pigment epithelium (RPE).