Figure 5. Calretinin and calbindin-28 kDa immunofluorescence is not present in the distal dendritic compartment of retinal ganglion cells as seen in retinal whole-mounts. A-C: Double labeling for calretinin (red) and microtubule-associated protein 1 (MAP-1; green) in the ganglion cell layer (GCL)/nerve fiber layer (NFL) shows that MAP-1 positive dendrites are not colabeled with calretinin. Some large retinal ganglion cells (RGCs) that are completely ringed by MAP-1 staining (arrowhead) are not positive for calretinin. RGCs with smaller somata partially ringed with MAP-1 staining are immunopositive for calretinin (arrows). Other brightly stained somata not showing MAP-1 immunofluorescence are the displaced amacrine cells that also stained for calretinin. D-F: Confocal plane showing that the MAP-1 positive (green) dendrites (arrow) do not merge with the calretinin-positive plexus (red) in the inner plexiform layer. G-I: Double labeling for calbindin-28 kDa (red) and MAP-1 (green) in the GCL/NFL shows that some large RGCs that are completely ringed by MAP1 staining (arrowhead) are positive for calbindin-28 kDa. Some RGCs with smaller somata that are incompletely ringed with MAP-1 are also calbindin-28 kDa positive while others are not (arrow). Some neurons incompletely ringed with MAP-1 are also not calbindin-28 kDa positive (arrow). Scale bar represents 20 µm.