Figure 3. Calretinin and calbindin-28 kDa are differentially distributed in amacrine cells as seen in retinal whole-mounts. A-C: Double labeling for calretinin (red) and calbindin-28 kDa (green) in the inner nuclear layer shows that labeling for each was present in a distinct set of amacrine cells For the apparent region of overlap (arrowhead) in the overlay of this confocal plane, examination of different z-planes revealed that these were disparate cells located at different depths. Arrows indicate calbindin-28 kDa-immunopositive cells. Scale bar represents 20 µm. Abbreviations: bv is blood vessel.