Figure 1. Schematic representation of calretinin and calbindin-28 kDa proteins and their sequence identity. A: Shown is a schematic representation of calretinin and calbindin-28 kDa proteins. The red blocks mark the E-helix-loop-F-helix-hand (EF) hand regions within each molecule. B: Alignment of the amino acid sequences of rat calretinin and calbindin-28 kDa molecules is based on NCBI accession numbers P47728 and P07171 respectively. Protein sequences were obtained from the NCBI protein database. C: Western blots for different calbindin-28 kDa (CB) and calretinin (CR) antibodies for rabbit (R) and mouse (M) are shown. Both calbindin-28 kDa and calretinin antibodies recognized a single protein band close to 26 kDa. The blot on the far right used antibodies for both calretinin (AB148) and calbindin-28 kDa (300). The arrow indicates the putative calbindin-28 kDa-positive band below the thicker calretinin positive band.