Figure 4. The effect of UV-B treatment on
the expression of Nalp subtypes in HCE-T cells. Changes of
Nalp
mRNA expression 6 h (
A) or 24 h (
B) after UV-B treatment
are shown. The relative expressions of
Nalp1,
Nalp2,
Nalp3,
Nalp7, and
Nalp10 were measured in 85%–90% confluent
HCE-T cells irradiated with 30 mJ/cm
2 UV-B as described in
the legend of
Figure
1 and are shown compared to cells incubated in the same way
but not irradiated. Mean values and ±SD were calculated from six
independent measurements. The asterisk indicates a p<0.005. Changes
of Nalp1 protein expression 6 h (
C) and 24 h (
D) after
UV-B irradiation of HCE-T cells are illustrated. Equal amount of sample
loading was verified by detecting β-actin protein expression.