Figure 8 of Raymond, Mol Vis 2008; 14:1559-1574.


Figure 8. CFP expression colocalizes with ChAT immunoreactivity, a marker of cholinergic amacrine and displaced amacrine cells in the inner retina. A: A transverse section of peripheral retina shows cyan fluorescent protein (CFP) in numerous cell bodies in the ganglion cell layer (GCL), and weakly-CFP-expressing cell bodies in the proximal inner nuclear layer (INL). B: Choline acetyltransferase (ChAT) immunoreactivity is in amacrine and displaced amacrine cell somata in the INL and GCL, respectively. C: A merged image of A and B shows the colocalization of CFP and ChAT immunoreactivity in small, weakly CFP-expressing cell bodies in the INL and GCL. The scale bar for A-C is 45 μm. D: A higher magnification image of transverse retina shows CFP in ganglion cells in the GCL and in smaller, weakly CFP fluorescent cells in the INL and GCL (arrows). E: Same section as in D shows ChAT immunoreactivity in numerous amacrine and displaced amacrine cell somata in the INL and GCL (arrows). F: A merged image of D and E shows the colocalization of CFP expression and ChAT immunoreactivity in amacrine and displaced amacrine cells (arrows) in the INL and GCL. The scale bar for D-F is 25 μm. G: CFP is localized to brightly and weakly fluorescent cell bodies of various sizes in the GCL. Image is from a retinal wholemount located 1.5 mm from the optic nerve head in midperipheral nasal retina. H: ChAT immunoreactivity is in displaced amacrine cell somata in the GCL. I: A merged image of G and H shows colocalization of CFP expression and ChAT immunoreactivity in displaced amacrine cell bodies in the GCL. J: CFP is localized to weakly fluorescent cell bodies in the INL. Image is from a retinal wholemount located 1.5 mm from the optic nerve head in midperipheral nasal retina. K: The same region as in J shows ChAT immunoreactivity in amacrine cell somata in the INL. L: A merged image of J and K shows the colocalization of CFP and ChAT immunoreactivity in amacrine cell bodies in the INL (arrows). There are also a few small CFP somata that do not contain ChAT immunoreactivity in the INL (stars). The scale bar for G-L is 45 μm.