Figure 7. CFP expression colocalizes with GAD₆₇ or GAT-1 immunoreactivity in the inner retina. A: A transverse section of peripheral retina shows cyan fluorescent protein (CFP) in numerous cell bodies in the ganglion cell layer (GCL), and weakly CFP-expressing cell bodies in the proximal inner nuclear layer (INL). B: The same region as in A shows L-glutamate decarboxylase 67 (GAD₆₇) immunoreactivity in amacrine and displaced amacrine cell somata in the INL and GCL, respectively. C: The merged image of A and B shows colocalization of CFP expression and GAD₆₇ immunoreactivity in small, weakly CFP-expressing cell bodies in the INL and GCL. The scale bar for A–C is 45 μm. D: A higher magnification image of transverse retina shows CFP in ganglion cell somata in the GCL and in smaller, weakly CFP fluorescent somata in the GCL and INL (arrows). E: GAD₆₇ immunoreactivity is in numerous amacrine and displaced amacrine cell somata in the INL and GCL (arrows). F: The merged image of D and E shows colocalization of CFP expression and GAD₆₇ immunoreactivity in amacrine and displaced amacrine cells (arrows) in the INL and GCL. The scale bar for D-F is 25 μm. G: CFP is localized to brightly and weakly fluorescent cell bodies of various sizes in the GCL. Image is from a retinal wholemount located 1.5 mm from the optic nerve head in midperipheral nasal retina. H: The same region as in G shows GABA plasma membrane transporter-1 (GAT-1) immunoreactivity near or at the plasma membrane of displaced amacrine cell somata in the GCL. I: Merged image of G and H shows colocalization of CFP expression and GAT-1 immunoreactivity in displaced amacrine cell somata in the GCL (arrows). Most GAT-1 immunoreactive somata lack CFP. J: CFP is localized to weakly fluorescent cell bodies in the INL. Image is from a retinal wholemount located 1.5 mm from the optic nerve head in midperipheral nasal retina. K: Same region as in J shows GAT-1 immunoreactivity in amacrine cell somata in the INL. L: Merged image of J and K shows colocalization of CFP expression and GAT-1 immunoreactivity in amacrine cell bodies in the INL. The scale bar for G-L is 45 μm. In C and F inner plexiform layer is abbreviated inner plexiform layer (IPL).