Figure 4. CFP-containing ganglion cells express NF-L immunoreactivity in their soma, dendrites and axons in the fiber layer, optic nerve head and optic nerve. A: Transverse section of peripheral retina shows cyan fluorescent protein (CFP) expression in numerous cell bodies in the ganglion cell layer (GCL). B: Same section as in A shows neurofilament light (NF-L) immunoreactivity in ganglion cell somata in the GCL and dendrites in the inner plexiform layer (IPL). C: A merged image of A and B demonstrates colocalization of CFP expression and NF-L immunoreactivity in many cell bodies in the GCL and dendrites in the IPL. The scale bar for A-C is 40 μm. D: A higher magnification image of transverse retina shows CFP fluorescence in ganglion cells, including large ganglion cell somata (arrows). E: NF-L immunoreactivity is evident in numerous cell bodies in the GCL including the large ganglion cell somata (arrows). F: A merged image of D and E demonstrates colocalization of CFP expression and NF-L immunoreactivity in most cell bodies in the GCL and dendrites in the IPL. The scale bar for D-F is 25 μm. G: CFP is localized to brightly and weakly fluorescent cell bodies of various sizes in the GCL. Image is from a retinal wholemount located 1.5 mm from the optic nerve head in midperipheral nasal retina. H: NF-L immunoreactivity is evident in ganglion cell somata in the same region as G. I: A merged image of G and H shows colocalization of CFP expression and NF-L immunoreactivity in ganglion cell bodies. The scale bar for G-I is 30 μm. J: CFP fluorescence is evident in ganglion cell axons in the fiber layer, optic nerve head and optic nerve, in this low magnification image of a transverse section through the optic nerve head. K: The same section as in J, shows NF-L immunoreactivity in ganglion cell axons. L: A merged image of J and K shows colocalization of CFP and NF-L immunoreactivity in most ganglion cell axons in the fiber layer, optic nerve head and optic nerve. Note: there is not complete overlap of CFP and NF-L in ganglion cell axons, as some NF-L labeled cells do not express CFP. The scale bar for J–L is 140 μm. In C and F inner nuclear layer is abbreviated INL, and outer plexiform layer is abbreviated OPL.