Figure 2. Comparison of cyan fluorescent
protein expression in the ganglion cell layer with DAPI-labeled retinal
neurons. A: Image of a retinal wholemount through the
midperipheral ganglion cell layer (GCL) showing that cyan fluorescent
protein (CFP) expression is localized to numerous cell bodies. B:
Image illustrates the same region as in A, showing DAPI
fluorescence in all cell nuclei in the GCL. C: A merged image
of A and B shows colocalization of CFP expression and
DAPI nuclear labeling in about half of the neuronal nuclei in the GCL.
Scale bar for A-C represents 50 μm. Graphs showing the
average densities of CFP- and DAPI-labeled cells in the GCL along the
dorsoventral (D) and nasotemporal (E) axes of five
retinal wholemounts from five different thy1-CFP transgenic mice. Error
bars are SEM.