Table 1 of Lesch, Mol
Vis 2008; 14:1549-1558.
Table 1. Sequences of primers used to amplify the coding regions of the RS1 gene.
Exon |
Primers (5′-3′) |
Product size (bp) |
Ta (°C) |
---|---|---|---|
1 | F: CTCAGCCAAAGACCTAAGAAC | 216 | 58 |
R: GTATGCAATGAATGTCAATGG | |||
2 | F: GTGATGCTGTTGGATTTCTC | 176 | 56 |
R: CAAAGTGATAGTCCTCTATG | |||
3 | F: CGATGCATAAGGACTGAGTGTGATC | 377 | 50 |
R: GCATTAACATAGGCTTAC TAATAG | |||
4 | F: CGTGAGTAGTGAACCGTTGAAGAC | 381 | 50 |
R: ACGCTGGTAGAGAGGCCTAT | |||
5 | F: GCAAGTTAAGTATAACGGAAGCTG G | 508 | 50 |
R: GGAAAGCGCAGATGATCCACTGTG | |||
6 | F: GCAAACTGCTTTAACTAC TTCC | 427 | 50 |
R: CCAGCACTGCAGTTACAATTGC |