Table 1 of Lesch, Mol
Vis 2008; 14:1549-1558. Table 1 of Lesch, Mol
Vis 2008; 14:1549-1558.
Table 1. Sequences of primers used to amplify the coding regions of the RS1 gene.
Exon |
Primers (5′-3′) |
Product size (bp) |
Ta (°C) |
|---|---|---|---|
| 1 | F: CTCAGCCAAAGACCTAAGAAC | 216 | 58 |
| R: GTATGCAATGAATGTCAATGG | |||
| 2 | F: GTGATGCTGTTGGATTTCTC | 176 | 56 |
| R: CAAAGTGATAGTCCTCTATG | |||
| 3 | F: CGATGCATAAGGACTGAGTGTGATC | 377 | 50 |
| R: GCATTAACATAGGCTTAC TAATAG | |||
| 4 | F: CGTGAGTAGTGAACCGTTGAAGAC | 381 | 50 |
| R: ACGCTGGTAGAGAGGCCTAT | |||
| 5 | F: GCAAGTTAAGTATAACGGAAGCTG G | 508 | 50 |
| R: GGAAAGCGCAGATGATCCACTGTG | |||
| 6 | F: GCAAACTGCTTTAACTAC TTCC | 427 | 50 |
| R: CCAGCACTGCAGTTACAATTGC |