Figure 6. γ-Synuclein specificity was examined using immunofluorescent staining of rat retinal cells. A: DAPI (blue) counterstained the nuclei. B: Brn-3a (green) was localized to RGC nuclei. C: γ-synuclein (red) was localized throughout the cytoplasm (arrowheads) and processes (arrows). A merged image of A, B, and C is shown in D. Many cells demonstrated neither Brn-3a nor γ-synuclein reactivity (horizontal arrows in A). A subset of presumptive retinal ganglion cells demonstrated intensive staining by both γ-synuclein and Brn-3a (full arrowheads in B, C, and D), while many other cells showed only weak staining by both γ-synuclein and Brn-3a (arrowheads in B and C). A few cells were stained by γ-synuclein, but not by Brn-3a (vertical arrows in B).