Figure 2. Localization of γ-synuclein was studied by immunohistochemistry in a normal human retina (A) and in the retina of an age-matched patient with retinoblastoma (B). γ-Synuclein was detected using a diaminobenzidine reagent (brown, peroxidase). Tissues were counterstained with hematoxylin (blue). Sections were stained with an antibody that recognized γ-synuclein, but not the other members of the synuclein family. The retina of the retinoblastoma patient (B) demonstrated more intense γ-synuclein immunoreactivity in retinal ganglion cell bodies (arrows) and less intensive staining in the nerve fiber layer (NFL) compared with the normal retina (A).