Figure 1. Effect of adoptive transfer of mast cells isolated from wild type mice to mast cell-deficient mice on the clinical responses in the acute phase. A: Recovery of defective acute phase clinical responses in Kit^w/Kit^w-v mice after adoptive subconjunctival transfer of conjunctival mast cells from wild type cells is shown. The clinical scores for both sensitized and mock-sensitized Kit^w/Kit^w-v mice were significantly lower following allergen challenge than that of wild type mice. B: Allergen-induced-mast cell degranulation in Kit^w/Kit^w-v mice after adoptive transfer of conjunctival mast cells from wild type mice is demonstrated in the chart. Mast cell degranulation in the conjunctiva, undetectable in Kit^w/Kit^w-v mice, was observed in both sensitized and mock-sensitized adoptive transfer mice. C: Expression of mast cell-restricted proteases, mMCP-5 and mMCP-6, in the conjunctiva of Kit^w/Kit^w-v mice after adoptive transfer is shown. Mice injected with the conjunctival mast cells had levels of mMCP-5 and mMCP-6 that were comparable to those of wild type mice. D: Expression of CXCR3 as a maturation marker on conjunctiva-derived FcεRI⁺ c-Kit⁺ mast cells by FACS analysis is demonstrated. Red indicates the CXCR3 stained conjunctiva-derived mast cells. Black shows the isotype control. RW: sensitized with ragweed pollen; PBS: mock sensitized; n=10 mice/group.