Figure 5. Immunohistochemical analysis of VEGF-A_165b expression in the glaucomatous retina after five days of elevated IOP. A-C: Negative control. Non-specific staining was not detected in the retina. D-F: VEGF-A_165b staining of the normal retina (n=4). VEGF-A_165b was present in the RGC and the INL. G-I: VEGF-A_165b staining of the glaucomatous retina (n=4). VEGF-A_165b staining was stronger in the RGC and the INL compared to the normal retina. J-L: Negative control. No non-specific staining was observed in the retina. M-O: VEGF-A_165b staining of the RGC in the normal retina. Staining was colocalized with the RGC marker, Fluorogold. P-R: VEGF-A_165b staining of the RGC in the glaucomatous retina. Levels of VEGF-A_165b in the Fluorogold-labeled RGC (white arrow) were increased in the retinas with elevated IOP.