Figure 3. Anti-VEGF-A165b
antibody does not recognize VEGF-A164.A:
Incubation with VEGF-A165b antibody. This antibody
recognizes the VEGF-A165b monomer (22.5 kDa) and dimer (45
kDa) in control and glaucomatous retinas (first two lanes). VEGF-A165b
antibody does not recognize 25 ng, 100 ng, or 250 ng of VEGF-A164
recombinant protein in the same membrane (last three lanes). B:
Incubation of the same membrane with the anti-VEGF antibody after
stripping. The anti-VEGF antibody recognizes VEGF-A164
recombinant protein at all concentrations.