Figure 2. Immunohistochemical analysis of VEGF-A₁₆₄ expression in the glaucomatous retina after five days of elevated IOP. A-C: Negative control. Some non-specific staining of blood vessels in the RGC and the INL was observed. D-F: VEGF-A₁₆₄ staining of the normal retina (n=4). VEGF-A₁₆₄ was present in the RGC and the INL. G-I: VEGF-A₁₆₄ staining of the glaucomatous retina (n=4). Staining was detected in the RGC and INL. VEGF-A₁₆₄ levels did not differ between the normal and glaucomatous retinas.