Figure 1. Western blot analysis of VEGF-A₁₆₄ expression in the glaucomatous retina. A: VEGF-A₁₆₄ was detected at 45 kDa in control and glaucomatous retinas after five days of elevated IOP. B: Similarly, VEGF-A₁₆₄ was observed at 45 kDa in the control and glaucomatous retinas following 10 days of elevated IOP. C: Glaucomatous/control ratio of normalized VEGF-A₁₆₄ densitometry readings in the retina is demonstrated in the chart. VEGF-A₁₆₄ was expressed at comparable levels in the control and glaucomatous retinas after five and 10 days of elevated IOP. The positive control was the brain, and the loading control was α-tubulin.