Figure 1. TUNEL positive cells can be detected in TM samples from glaucomatous patients. TM samples were stained with DAPI (A,B) and by TUNEL (C). The DAPI staining allowed us to visualize the structure of the specimen and to identify apoptotic nuclei both within and outside of the TM. Dotted lines represent the limits of the TM and the arrows indicate the presence of apoptotic cells in the TM and sclera of a POAG patient. The inset in C shows a pyknotic nucleus (visualized by DAPI blue staining) that is also positive for TUNEL (green). Abbreviations in the figure are Co, Cornea; TM, Trabecular Meshwork; Sc, Sclera. The scale bar is equal to 250 μm in A and 150 μm in B and C.