Figure 9. Inhibition of cell attachment by transforming growth factor, beta-induced protein. Solid-phase adhesion assay of primary human scleral fibroblasts to wells pre-coated with 0-25 μg bovine serum albumin (BSA), fibronectin (FN), and transforming growth factor, beta-induced protein (TGFBIp) on poly-D-lysine 96-well plates. Cells were seeded 2000 cells/well and allowed to attach for 45 min in serum-limiting media. Unattached cells were gently rinsed off and remaining attached cells were quantified by measuring hexosaminidase, as described in Methods. Cell attachment was significantly decreased in wells coated with 25 μg TGFBIp as compared to BSA controls. Data are expressed as the mean±SEM by the Student t-test for unmatched pairs for three individual experiments in triplicate. In the figure, the asterisk indicates p≤0.05.