Figure 4. Volcano plot of marmoset choroid/RPE microarray data. Scatter plot represents a summary of the t-tests for the individual genes. For each gene this plot shows log₂ of fold change between the mean expression in minus lens-treated group and plus lens-treated group as plotted against −log₁₀ (p), where p is a probability value (for a given gene) which is associated with the Student t-test comparison of the two groups of samples. Using a p value of 0.05 as the cutoff threshold, 21 genes were significantly downregulated in minus lens-treated eyes as compared with plus lens-treated eyes (negative log₂) and 183 genes were significantly uppregulated in minus lens-treated eyes as compared with plus lens-treated eyes (positive log₂). Each point represents an individual gene in the array; genes not significantly altered are depicted in black, whereas genes significantly changed are indicated in red. Those genes appearing on the upper left or right region have a large fold-change and a smaller p value.