Figure 1 of Zhao, Mol Vis 2008; 14:1456-1464.

Figure 1. The frequency of IFN-γ producing cells detected by ELISPOT assay. Fresh PBMCs were cultured with mixed S-Ag peptides plus anti-CD28 mAb. PBMCs cultured with the medium and anti-CD3 plus anti-CD28 mAbs, respectively, were used as negative and positive controls. If the value of IFN-γ SFC was more than 2, then it was considered a positive well. Upon S-Ag peptides stimulation, 13 active BD patients and three inactive BD patients showed a positive response and the frequency of S-Ag specific T cells was similar between the two groups of patients (p>0.05, Student’s t-test). Upon anti-CD3 stimulation, all the subjects responded positively and the number of IFN-γ SFC was larger than that stimulated by S-Ag peptides. The frequencies of IFN-γ producing cells in four groups of PBMCs upon anti-CD3 stimulation were analyzed by ANOVA. Significant statistical difference was set at p<0.05 (*) and p<0.01 (#); ns means not significant while bars represent mean values.