Figure 6. Western blot showing the effect of inhibition of the CPEB antibody with PEP-171 neutralizing peptide. Equal concentrations of total retinal protein was isolated from control octopi at 180 min of dark or light adaptation and from octopi moved to the opposite lighting conditions at 5 min, 15 min, and 45 min time intervals. Lanes 2-5 and 7-10: The signal of the two major immunoreactive bands present between 60-80 kDa is remarkably reduced, as is the signal of smaller band is detected below 30 kDa. The molecular weight sizes correspond to the fragments of the Magic Mark XP protein standard (Invitrogen) in lanes 1 and 6. The following abbreviations are used in the figure: standard (Std), light-adapted (LA), and dark-adapted (DA).