Figure 4. CPEB protein is detected in dark-adapted and light-adapted octopus retinas. Equal concentrations of total retinal protein were isolated from control octopi at 180 min of dark or light adaptation and from octopuses moved to the opposite lighting conditions at 5 min, 15 min, and 45 min time intervals. Lanes 2-5 and 7-10: Two major immunoreactive bands are present between 60-80 kDa (single asterisk), while a smaller one is detected below 30 kDa (double asterisk). The molecular weight sizes correspond to the fragments of the Magic Mark protein XP standard (Invitrogen) in lanes 1 and 6. The following abbreviations are used in the figure: standard (Std), light-adapted (LA), and dark-adapted (DA).