Figure 2. Agarose gel electrophoresis of α-tubulin 3’-RACE. Equal concentrations of mRNA used for 3’-RACE was isolated from control octopuses at 180 min of dark or light adaptation and from octopi moved to the opposite lighting conditions at 5 min, 15 min, and 45 min time intervals. Lanes 2-5 and 9-12: The asterisk indicates the approximate size of the amplified α-tubulin 3’-RACE fragment as 325 bp. The DNA marker sizes correspond to the fragments of the low molecular weight ladder (New England BioLabs Inc., Ipswich, MA) in lanes 6 and 8. Lanes 1 and 13 contain the 1 kb DNA ladder (New England BioLabs Inc.), and lane 7 contains the 100 bp DNA ladder (Invitrogen, Carlsbad, CA). The following abbreviations are used in the figure: marker (M), light-adapted (LA), and dark-adapted (DA).