Figure 1. Multiple alignments using ClustalW and amino acid conservation of four novel missense sequence variations identified in RPGRIP1: c.1295C>T (S432F), c.1802C>G (S601W), c.1892A>T (H631P), and c.3170A>T (H1057L). The first six amino acid sequences in each segment represent RPGRIP1 proteins of several species, and the last four denoted with (L) represent RPGRIP1-like proteins. Alignment results show that histidine at codon 631 is highly conserved, but amino acids at codon 432, 601, and 1057 are poorly conserved.