Figure 13. Reverse transcriptase
polymerase chain reaction amplification of EMT-associated transcription
response elements mRNAs during ARPE-19 and chick embryo retinal pigment
epithelium cell differentiation RNA was isolated from undifferentiated
and differentiated ARPE-19 or chick embryo retinal pigment epithelium
(cRPE) cells and subjected to RT–PCR analysis to detect transcription
response elements (TRE) mRNAs as described in Methods. Lanes 1 and 16
represent DNA standard ladders at 300, 400, and 500 bp, lanes 8 and 15
represent the positive controls for GADPH, and the remaining lanes
represent primers specific for the following TFs: 2 and 9, Slug; 3 and
10, Snail; 4 and 11, Twist; 5 and 12, SIP-1; 6 and 13, SMAD-2; 7 and
14, LEF-1.