Figure 12. Reverse transcriptase polymerase chain reaction amplification of transcription response elements mRNAs during chick embryo retinal pigment epithelium cell differentiation RNA was isolated from undifferentiated cultured chick embryo retinal pigment epithelium (RPE) cells or differentiated fresh RPE tissue and subjected to RT–PCR analysis to detect transcription response elements (TREs) mRNAs as described in Methods. Lanes 1 and 11 represent DNA standard ladders at 300, 400, 500, 600, and 700 bp, lane 10 represents the positive control for GADPH, and the remaining lanes represent primers specific for the following TFs: 2 FoxD3, 3 AML-1, 4 HNF-3α, 5 HNF-1, 6 E2F1, 7 DP1, 8 TFII-I, 9 SREBP-1.