Figure 4. Localization of α-smooth muscle
actin (SMA) and the absence of lymphatic endothelium in scarred
filtering bleb tissue. Serial sections were double labeled for
TGF-β-RII (A, D, and G; green) and α-SMA (B,
C), O-linked sialoglycoprotein (E, F), and
PECAM-1 (H, I, red). α-SMA is colocalized to TGF-β-RII
in stromal fibroblasts and perivascular cells (A,B,C).
Vascular structures expressing TGF-β-RII(H) were negative for
markers of lymphatic endothelium (E).Some collagen fiber
autofluorescence is present in the red channel (B, E, H),
Scale bar represents 50 µm.