Figure 3. Chromatograms showed by the analysis of the serial dilutions by the dHPLC. The percentage data indicate the amount of the carrier DNA present in the dilution. The double-peak corresponding to the heteroduplexes decreases in size as the percentage of mutant DNA in the sample gets lower. From the 5% to the 2% dilution, the double-peak becomes a slight peak (pointed by the arrows in the figure). Below the 2%, the heteroduplexes could not be distinguished. These chromatograms revealed that the mutation was detectable up to the 2% dilution.