Figure 2. Chromatograms of the control DNAs obtained by dHPLC. After PCR amplification of exon 8 of CRB1 (where the p.Cys896ter mutation is located), the PCR products were analyzed by dHPLC. A: Electropherogram showed by the paternal DNA (carrier for the p.Cys896ter mutation). 1: Double-peak generated by the heteroduplexes forms associated to the presence of the p.Cys896ter mutation in the sample. 2: Peak generated by the homoduplexes. The presence of both homo and heteroduplexes peaks indicates a heterozygous genotype for the p.Cys896ter mutation. B: Electropherogram showed by the maternal DNA (wild-type for the p.Cys896ter mutation). 2: Peak generated by the homoduplexes. The only presence of homoduplexes peak represents a wild-type genotype.