Figure 1. Ethidium bromide-stained 2%
agarose gels and direct sequencing, showing the PCR-restriction
fragment length polymorphism and corresponding sequence chromatogram.
A:
Restriction analysis for complement factor H (
CFH) Y402H
polymorphism resulted in digestible fragment (T/T), undigestible
fragment (C/C), and heterozygote (T/C).
B: Direct sequencing of
the PCR products confirmed the restriction patterns for CFH Y402H.
C:
Restriction analysis for
LOC387715rs10490924
resulted in digestible fragment (G/G), undigestible fragment (T/T), and
heterozygote (T/G).
D: Sequencing confirmed the restriction
patterns for
LOC387715rs10490924.
E: Restriction analysis for
HTRA1rs11200638
resulted in digestible fragment (G/G), undigestible fragment (A/A), and
heterozygote (G/A).
F: Sequencing confirmed the restriction
patterns for
HTRA1rs11200638.