Molecular Vision: Julien, Mol Vis 2008; 14:1358-1371. Table 2

Table 2. Different methods used to induce CNV in rabbits

Methods	Fluorescein angiography	Choroidal neovascularization (CNV)	Fenestrations of newly formed vessels detected by EM	RPE	Inflammation	Reference
Subretinal injection of vitreous	Negative, no leakage	Subretinal vessels in 33%–55% of the eyes after 4–40 weeks	Normal fenestration	Migration; proliferation	n.d.	[34]
Retinotomy with argon laser burns	Not clinically apparent subretinal neovascularization	Microscopical subretinal neovascularization	Normal fenestration	Disruption	Macrophages	[33]
VEGF pellets into the vitreous	Leakage in retinal vessels between 7 and 14 days	n.d.	n.r.;electron micrographs of immature vessels do not show fenetrations	n.d.	n.r.	[25]
Subretinal injection of bFGF micropheres	n.d.	Neovascular channels up to 8 weeks	n.d.	Migration; proliferation	Macrophages appeared early but then disappeared	[30,39]
Subretinal implantation of microsheres impregnated with FGF	CNV leakage in the eyes afer 4 weeks	Subretinal vessels in neovascular membranes	n.d	Migration; proliferation	Macrophages	[40]
Subretinal injection of lipide hydroperoxide	After 100 µg 9%–15% after 4 weeks	Histologically detected in 46% of the eyes	Normal fenestration	Detachment, damage	Macrophages	[32]
Subretinal injection of FGF-2 and LPS in Heparin-Sepharose beads	CNV leakage in 100% of the eyes afer 2 weeks and 3 months	CNV in all injectied eyes	n.d	Proliferation	Relationship of Makrophages to CNV	[29]
Subretinal injection of matrigel alone or in combination with VEGF	CNV leakage in 100% of the eyes afer 2 - 4 weeks also without VEGF	CNV in 100% of the eyes afer 2 - 4 weeks also without VEGF	n.d.	Displacement, vacuolisation, depigmentation	Many inflammatory cells	[26]
Injection of AdVEGF-A into the vitreous	n.d.	n.r.	n.d.	n.r.	Strong inflammatory reaction	[27]
Subretinal injection of HC-AdVEGF-A	CNV leakage in 83% of the eyes after 2–4 weeks (5x10⁶ i.u.)	Observed after 2–4 weeks	Not found in newly formed vessels	Migration; proliferation	Few macrophages	Present study