Table 2 of Julien, Mol Vis 2008; 14:1358-1372.

Table 2. Different methods used to induce CNV in rabbits

Methods Fluorescein angiography Choroidal neovascularization (CNV) Fenestrations of newly formed vessels detected by EM RPE Inflammation Reference
Subretinal injection of vitreous Negative, no leakage Subretinal vessels in 33%–55% of the eyes after 4–40 weeks Normal fenestration Migration; proliferation n.d. [34]
Retinotomy with argon laser burns Not clinically apparent subretinal neovascularization Microscopical
subretinal neovascularization Normal fenestration Disruption Macrophages [33]
VEGF pellets into the vitreous Leakage in retinal vessels between 7 and 14 days n.d. n.r.;electron micrographs of immature vessels do not show fenetrations n.d. n.r. [25]
Subretinal injection of bFGF micropheres n.d. Neovascular channels up to 8 weeks n.d. Migration; proliferation Macrophages appeared early but then disappeared [30,39]
Subretinal implantation of microsheres impregnated with FGF CNV leakage in the eyes afer 4 weeks Subretinal vessels in neovascular membranes n.d Migration; proliferation Macrophages [40]
Subretinal injection of lipide hydroperoxide After 100 µg 9%–15% after 4 weeks Histologically detected in 46% of the eyes Normal fenestration Detachment, damage Macrophages [32]
Subretinal injection of FGF-2 and LPS in Heparin-Sepharose beads CNV leakage in 100% of the eyes afer 2 weeks and 3 months CNV in all injectied eyes n.d Proliferation Relationship of Makrophages to CNV [29]
Subretinal injection of matrigel alone or in combination with VEGF CNV leakage in 100% of the eyes afer 2 - 4 weeks also without VEGF CNV in 100% of the eyes afer 2 - 4 weeks also without VEGF n.d. Displacement, vacuolisation, depigmentation Many inflammatory cells [26]
Injection of AdVEGF-A into the vitreous n.d. n.r. n.d. n.r. Strong inflammatory reaction [27]
Subretinal injection of HC-AdVEGF-A CNV leakage in 83% of the eyes after 2–4 weeks (5x106 i.u.) Observed after 2–4 weeks Not found in newly formed vessels Migration; proliferation Few macrophages Present study