Figure 1. In vitro biological activity of high capacity adenovirus vector encoding for vascular endothelial growth factor A¹⁶⁵ (HC Ad.VEGF-A¹⁶⁵) on human umbilical vascular endothelial cells (HUVEC). A: HUVEC proliferation was measured in response to supernatants (SN) from ARPE-19 cells transduced with HC Ad.VEGF-A¹⁶⁵ (MOI 20) with vascular endothelial growth factor (VEGF) protein concentrations as indicated. Blind means without HUVEC cells and control refers to medium without VEGF and bFGF. B: HUVEC migration was quantified in response to ARPE-19 cell supernatant (VEGF concentration 162 ng/ml), or commercial VEGF-A as a positive control. Each experiment was performed in triplicate and repeated on at least three occasions.