Table 1 of Zenteno, Mol Vis 2008; 14:1353-1357.
Table 1 of Zenteno, Mol Vis 2008; 14:1353-1357.
Table 1. Oligonucleotide sequences used for PCR amplification and direct automated nuclotide sequencing of WFS1 in this study.
| Exon | Primers (5’-3’) | Tm °C |
|---|---|---|
| 2 | F: GCAGACACTAAGTGCCAGA | 56 |
| R: CTGAACTGCAGAGGACCTG | ||
| 3 | F: GCAGCAGCAGATCTGAAGA | 56.6 |
| R: TCTCAGGCACCGACACTTCT | ||
| 4 | F: GAAGTGGGTGAAAGGAGGT | 53.9 |
| R: CAGTTAGCAAGCAGCATTAC | ||
| 5-6 | F: GTCAGAGTGGCACCGAAAGC | 65.1 |
| R: TCGCCCTGCAGGTGCAGGCTGGGA | ||
| 7 | F: ATTGCTCTGTGTGAGGGTG | 62.8 |
| R: CCTGCCTGAGGTGCGCGAGT | ||
| 8-1 | F: TTGCCCAGAGGCAGGGTGGT | 61.3 |
| R: ATGGAGGGCAGCAGCGATAGCA | ||
| 8-2 | F: ATCCCCTGCTCGGAGCTGGCT | 63 |
| R: AGTTGTAGACCTTCATGCC | ||
| 8-3 | F: CAAGGCCAGCTTCTCTGTGGT | 59.1 |
| R: ATGGCCTTGAGCTCGAAGACA | ||
| 8-4 | F: CAAGGACATCGTGCTGCGGGC | 63 |
| R: AGTCTGCACACGTGGGCACA |