Figure 1. Determination of cell proliferation in dexamethasone-exposed human lens epithelial cells. Quantitative determination of cell proliferation showed an increase in the number of Ki-67 positive cells in human lens epithelial cells exposed to 0.1 µM dexamethasone for 24 h whereas higher concentrations of dexamethasone did not significantly affect cell proliferation. At least 300 cells in three different fields were counted. The mean and SEM were calculated from three separate chamber slides (n=3). The experiment was repeated twice with different cell lines, obtaining similar results.