Figure 1. Electrophoresis of polymerase chain reaction-restriction fragment length polymorphism products for detection of nucleotide alterations of rs1048661 and rs3825942. DNA fragments with G at codon 141 (rs1048661) contained the Apa I restriction site, resulting in fragments of 304 bp+264 bp, while DNA fragments with T lacked the Apa I restriction site, resulting in a fragment of 568 bp. DNA fragments with A at codon 153 (rs3825942) contained the Hinf I restriction site, resulting in fragments of 335 bp+233 bp, while DNA fragments with G lacked the Hinf I restriction site, resulting in a fragment of 568 bp. Information for rs1048661: M; 100 bp marker, Lane 1; H₂O (negative control), Lane 2; GG (positive control), Lane 3; TT (positive control), Lane 9 and 10; GG, Lane 5, 6, and 8; TG, Lane 4 and 7; TT. Information for rs3825942: M; 100 bp marker, Lane 1; H₂O (negative control), Lane 2; AG (positive control) Lane 3; GG (positive control), Lane 5, 6, and 7; GG, Lane 4, 8, and 10; AG, Lane 9; AA.