Figure 2. Representative laser scanning microscopy images of HeLa cells co-transfected with the positive controls, GFP-αA and RFP-αB. The constructs were co-transfected into HeLa cells. After culture, laser scanning microscopy (LSM) images were taken. αA- and αB-crystallins are known to have a strong subunit-subunit interaction. The energy transfer efficiency is high. The increase of GFP fluorescence intensity is converted to pseudocolor (right panel) that displays variations of pixel gray scales with color.