Figure 1. Representative laser scanning microscopy images of HeLa cells transfected with GFP-VIM and co-transfected with GFP-VIM and WT αB-crystallin (αB) or R120G αB-crystallin (αBM). The single construct or pair of constructs was transfected into HeLa cells. After culture, laser scanning microscopy (LSM) images were taken. Either the green image (GFP-VIM) or merged image of green and red fluorescence (GFP-VIM and RFP-WT αB-crystallin [αB] or GFP-VIM and RFP-R120G αB) was shown. Vimentin filaments are clearly shown in cells co-expressing WT αB-crystallin, but enormous aggregates were formed in the cells co-expressing R120G αB-crystallin. Vimentin filaments are shown as the fibrous structures and aggregates as the bright, dense spots.