Figure 5. Fibroblast cell culture from the conjunctival biopsy of the proband. A: Fibroblastic cellular outgrowth from the conjunctival biopsy tissue. B: Following one week of cell culture, cellular confluence of fibroblasts is achieved. C: Following the addition of filipin stain, there is no evidence of cholesterol in the cultured conjunctival fibroblasts. D: Cells cultured with the addition of low density lipoprotein (LDL), acted as a positive control showing evidence of cholesterol with filipin staining. (Bar=100 microns).